Research Paper Volume 11, Issue 10 pp 3298—3314

GDF10 inhibits proliferation and epithelial-mesenchymal transition in triple‐negative breast cancer via upregulation of Smad7

Figure 3. Downregulation of GDF10 promotes proliferation of MDA-MB-231 cells. GDF10 expression at the mRNA (A) and protein (B) levels after transfection with non-coding negative control shRNA (NC), GDF10-shRNA1, and GDF10-shRNA2. *P < 0.05, **P < 0.01, compared with the NC group. (C) Cell proliferation assay. MDA-MB-231 cells were transfected with NC, GDF10-shRNA1, and GDF10-shRNA2 and proliferation measured with the CCK-8 assay at 0, 24, 48, and 72 h. *P < 0.05, **P < 0.01, compared with the NC group. (D) Quantification of Ki67 expression by immunofluorescence in MDA-MB-231 cells. **P < 0.01, compared with the NC group. (E) Cell invasion assay. MDA-MB-231 cells were transfected with NC or GDF10-shRNA1 for 72 h and cell invasion assessed in Matrigel-coated transwell inserts. **P < 0.01, compared with the NC group.