Figure 5. circFADS2 targets miR-498. (A) Bioinformatic analysis indicating that miR-498 has a circFADS2 binding site. (B) DLRA was performed following co-transfection of chondrocytes with a luciferase reporter containing either a WT (wild-type) or MU (mutant) circFADS2 and a miR-498 mimic. (C) qPCR analysis showing the decreased miR-498 expression levels in RA patients. (D) Chondrocytes were transfected with si-circFADS2 or si-NC and then treated with LPS. qPCR analysis was used to measure the miR-498 levels in each group. (E) Quantification of miR-498 and GAPDH mRNA by northern blot analysis. *P < 0.05, **P < 0.01 vs. control cells; # P < 0.05 vs. LPS group.