Research Paper Volume 11, Issue 10 pp 3348—3361

circFADS2 protects LPS-treated chondrocytes from apoptosis acting as an interceptor of miR-498/mTOR cross-talking

Figure 6. miR-498 inhibitor reversed the effects of circFADS2 on LPS-treated chondrocytes. LPS-treated chondrocytes were co-transfected with si-circFADS2 and miR-498 inhibitor/miR-NC inhibitor. (A) qPCR analysis was performed to confirm the miR-498 levels in each group. (B, C) The mRNA and protein expression of the indicated proteins was determined by qPCR and WB. (D) The IL-6 level in cells was examined by ELISA. Results were normalized to GAPDH expression. *P < 0.05, **P < 0.01 vs. LPS group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. LPS+circFADSi+miR-NCi group.