Research Paper Volume 11, Issue 10 pp 3348—3361

circFADS2 protects LPS-treated chondrocytes from apoptosis acting as an interceptor of miR-498/mTOR cross-talking

Figure 8. miR-498 targets the mTOR. (A) Bioinformatic analysis showing that miR-498 has a binding site in the 3′-UTR of mTOR. (B) DLRA was performed following co-transfection of chondrocytes with a luciferase reporter containing either a WT (wild-type) or MU (mutant) mTOR and a miR-498 mimic. (C) qPCR analysis showing the significantly increased mTOR expression levels in RA patients. (D, E) Chondrocytes were transfected with si-circFADS2 or miR-498 mimic and then treated with LPS. qPCR and WB analysis were carried out to detect mTOR levels in each group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control cells; #P < 0.05, ###P < 0.001 vs. LPS group.