Figure 4. Circ_0132266 serves as a sponge for miR-337-3p. (A, B) FISH and nuclear and cytoplasmic fraction experiments were performed to detect the location of circ_0132266. (C) RIP assay performed with AGO2 antibody to assure the ability of circ_0132266 to serve as miRNA sponge. (D) Schematic showed the predicted binding sites between circ_0132266 and miR-337-3p and Sanger sequencing confirmed the accuracy of conducted vectors which will be used in the dual-luciferase assay. (E) The binding between circ_0132266 and miR-337-3p was verified through dual-luciferase activity assay.