Figure 1. Confirmation of MSC properties. (A) Mouse MSC surface markers (Sca-1, CD90, CD105, CD34, CD45 and HLA-DR) were examined by flow cytometry. Y-axis is the number of the cells, and the large scale is 105. X-axis is the level of the examined gene, and the large scale is a relative density value. (B) Differentiation assay for MSCs into osteocytes followed by Von kossa staining (left), into adipocytes followed by Oil red O staining (middle), and into chondrocytes followed by alcian blue staining (right). N=5. Scale bars are 50 μm.