Figure 5. Injection of TNF-α antagonizes MSC-induced M2-polarization of macrophages in MI-mice. (A–D) The injured heart at 4 weeks after transplantation was digested, and the dissociated cells were analyzed by F4/80 and CD163, shown by representative flow charts (A), by quantification (B), and by ELISA for arginase and iNOS (C), or analyzed by CD4 and CD8, shown by representative flow charts (D), and by quantification (E). In panel A, Y-axis is F4/80 staining, and the X-axis is CD163 staining. In panel C, Y-axis is CD4 staining, and the X-axis is CD8 staining. *p<0.05. NS: non-significant. N=5.