Research Paper Volume 11, Issue 11 pp 3716—3730

Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis

Figure 1. Expression level of MEG3 was upregulated in DN. (A) Blood glucose level, (B) urinary albumin excretion rate, (C) representative kidney histology of HE staining, and (D) DN markers (Col-4 and FN) in kidney sections in negative control and DN. (E) MEG3 expression was upregulated in DN tissues relative to that in the negative control; (F) MEG3 expression was measured in MCs stimulated with D-glucose at 5.5 (NC), 19.5 (LG), and 25 mmol/L (HG); (G) MEG3 expression was measured in MCs stimulated with 25 mmol/L glucose for 0, 12, 24, and 36 h; (HI) qPCR and FISH results showed that MEG3 was mainly expressed in the cytoplasm of MCs.