Figure 2. Activation of ALDH2 attenuates hyperoxia-induced increase in cytochrome c expression in lung vascular endothelial cells. (a) Whole cell lysates extracted from HMVEC cultured under different conditions (normoxia, 48 hrs of hyperoxia, or Alda-1 pretreatment followed by 48 hrs of hyperoxia) were evaluated for cytochrome C levels by Western blotting. Equal amounts of protein (20µg) were loaded per each lane. (b) Expression of Cytochrome C was normalized to β-actin and presented in arbitrary units. The results are shown in mean ± SEM (n=3). Data presented are representative of two independent experiments.