Figure 3. ALDH2 activation via Alda-1 attenuates hyperoxia-induced increase in Bax expression in lung vascular endothelial cells. (a) Whole cell lysates extracted from HMVEC cultured under different conditions (normoxia, 48 hrs of hyperoxia, or Alda-1 pretreatment followed by 48 hrs of hyperoxia) were evaluated for Bax levels by Western blotting. Equal amounts of protein (20µg) were loaded per each lane. (b) Expression of Bax was normalized to β-actin and presented in arbitrary units. The results are shown in mean ± SEM (n=3). Data presented are representative of two independent experiments.