Figure 4. ALDH2 activation through Alda-1 pretreatment enhances ALDH2 expression in lung vascular endothelial cells under hyperoxia. (a) Whole cell lysates extracted from HMVEC cultured under different conditions (normoxia, 48 hrs of hyperoxia, or Alda-1 pretreatment followed by 48 hrs of hyperoxia) were evaluated for ALDH2 levels by Western blotting. Equal amounts of protein (20µg) were loaded per each lane. (b) Expression of ALDH2 was normalized to β-actin and presented in arbitrary units. The results are shown in mean ± SEM (n=3). Data presented are representative of three independent experiments.