Research Paper Volume 11, Issue 11 pp 3832—3850

Knockdown of angiopoietin-like 2 induces clearance of vascular endothelial senescent cells by apoptosis, promotes endothelial repair and slows atherogenesis in mice

Figure 1. Age-dependent increase of senescence-associated p21, Pai-1 and angptl2 expressions in the native endothelium parallels plaque growth in the aorta. (A) mRNA expression of indicated genes was quantified in the native aortic endothelium of 3-mo (n=4), 5-mo (n=4), 6-mo (n=4) and 12-mo (n=4) control ATX mice. The average level of gene expression in 3-mo ATX mice was arbitrarily set at 1. Plaque area was quantified from longitudinally open thoracic aortas of 3-mo (n=7), 5-mo (n=5), 6-mo (n=7), 9-mo (n=12) and 12-mo (n=4) ATX mice. Data are expressed as mean±SEM. *: p<0.0001 vs. 3-mo ATX mice. (B) Representative pictures of age-related increase in atherosclerotic plaque in 3-, 6-, 9- and 12-mo ATX mice.