Research Paper Volume 11, Issue 11 pp 3832—3850

Knockdown of angiopoietin-like 2 induces clearance of vascular endothelial senescent cells by apoptosis, promotes endothelial repair and slows atherogenesis in mice

Figure 5. Time-dependent decrease of senescent-associated Angptl2 and p21 mRNA expressions inthe native endothelium of the aorta post-AAV1-shAngptl2 injection. Decrease of Angptl2 (A) and p21 (B) mRNA expression post-injections (1-week, n=5-6; 2-week, n=5-7; 4-week, n=4-7; 3-month, n=6-4) in the native endothelium freshly harvested from thoracic aortas of ATX mice treated with AAV1-shSCR or AAV1-shAngptl2. Average gene expression level in shSCR group was arbitrarily set at 1. Data are mean±SEM. *: p<0.05; **: p<0.01; ***: p<0.001 vs. shSCR group, at each time point.