Figure 3. AGTR1 promotes tumor cell proliferation, migration and invasion. (A) RT-PCR and (B) Western blot analysis of AGTR1 overexpression after transfection into MDA-MB-231 and MCF7 cells. One representative Western blot image is shown, and the quantification of AGTR1 levels is provided in the right panels. *** P<0.001, **** P<0.0001. (C) and (D) MOCK and AGTR1high cell viability after treatment with different concentrations of LOS (0, 100, 200, 300 μM) by CCK8. * P<0.05, ** P<0.01, *** P<0.001. (E) and (F) Effect of AGTR1 overexpression on cell migration and invasion. Images were captured with an inverted microscope (×100 magnification). The total number of migrating and invading cells in the fields was counted and is shown in the bottom panel. * P<0.05, ** P<0.01, *** P<0.001.