Research Paper Volume 11, Issue 12 pp 3969—3992

AGTR1 promotes lymph node metastasis in breast cancer by upregulating CXCR4/SDF-1α and inducing cell migration and invasion

Figure 4. AGTR1 contributes to lymph node metastasis via CXCR4. (A) Growth curve of subcutaneous xenografts with MOCK and AGTR1high-MDA-MB-231 cells in nude mice. AMD3100 was administrated i.p. 14 days after implanting AGTR1high-MDA-MB-231 tumors. * P<0.05, ** P<0.01. (B) and (C) Representative images of xenograft models and lymph nodes are shown by BLI; n=8. (D) Representative HE staining figures of non-metastatic or metastatic lymph nodes in 4T1 mice. (E) Quantification of the signal intensities of tumors and lymph nodes and rates of lymph node metastasis in MDA-MB-231 tumors. * P<0.05, ** P<0.01, **** P<0.0001. (F) Representative xenograft tissues in three groups, which were subjected to immunohistochemical staining with anti-CXCR4. HSCORE of CXCR4 protein expression in mouse tumor tissues. * P<0.05, **** P<0.0001.