Figure 5. AGTR1 increases proliferation, migration and invasion through CXCR4. (A) RT-PCR and (B) Western blot analysis of CXCR4 knockdown in AGTR1high cells. Representative pictures of Western blot of AGTR1 and CXCR4 expression and protein band intensities are shown. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. (C) Effect of AGTR1 overexpression and siCXCR4 on AGTR1high cell proliferation using CCK8. * P<0.05, ** P<0.01. (D) Effect of AGTR1 overexpression and siCXCR4 on cell migration and invasion. The total numbers of migrating and invading cells in the fields were counted and are shown in the bottom panel. * P<0.05, ** P<0.01.