Research Paper Volume 11, Issue 12 pp 3969—3992

AGTR1 promotes lymph node metastasis in breast cancer by upregulating CXCR4/SDF-1α and inducing cell migration and invasion

Figure 6. AGTR1 induces the expression of FAK/RhoA signaling molecules through CXCR4. (A) Effects of LOS and AGTR1 overexpression on CXCR4 expression in MDA-MB-231 and MCF7 cells detected by Western blot assay. Representative images are shown; protein bond intensities are in the right panel. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. (B) F-actin polymerization analysis in MDA-MB-231 stimulated with 100 nM SDF-1a or SDF-1a pretreated with LOS by rhodamine-phalloidin immunofluorescence. (C) Correlation between AGTR1 and CXCR4 analyzed by the TCGA database. (D) RT-PCR analysis of knocking down AGTR1 on MDA-MB-231 and MCF7 cells. *** P<0.001. (E) Representative figures of CXCR4 protein levels in siAGTR1 cells, as determined by Western blot assay. Quantification of AGTR1 and CXCR4 levels is in the right panel. *** P<0.001, **** P<0.0001.