Research Paper Volume 11, Issue 12 pp 3969—3992

AGTR1 promotes lymph node metastasis in breast cancer by upregulating CXCR4/SDF-1α and inducing cell migration and invasion

Figure 7. The instrumental role of CXCR4 in AGTR1-mediated cell migration through the FAK/RhoA pathway. (A) Effects of LOS and AGTR1 on the expression of FAK, p-FAK, RhoA, ROCK1, and ROCK2 analyzed through Western blotting. Representative images and protein bond intensities are provided in the bottom panel. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. (B) Effects of CXCR4 knockdown on the expression of FAK, p-FAK, RhoA, ROCK1, ROCK2, MLC and p-MLC in AGTR1high cells, as determined by Western blotting. Representative images and protein bond intensities are provided in the bottom panel. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001.