Figure 1. A large-scale CRISPR knockout screen for cellular senescence bypass. (A) Design of the senescence-associated sgRNA library. (B) Schematic diagram of the sgRNA library construction and the CRISPR knockout screen. (C) Scatterplot of the normalized reads count of all sgRNAs in control and bleomycin-induced samples. sgRNAs targeting positive control genes (TP53 and CDKN1A) and negative control genes were shown in different colors. (D) The source of the candidate senescence bypass genes in the design approach of the senescence-associated sgRNA library.