Research Paper Volume 11, Issue 12 pp 4032—4049

Long-time qingyan formula extract treatment exerts estrogenic activities on reproductive tissues without side effects in ovariectomized rats and via active ER to ERE-independent gene regulation

Figure 5. The effects of QYFE on expression of PCNA or Ki67 in the uterus, vagina and mammary gland in OVX rats. PCNA and Ki67 expression was assessed by quantitative immunohistochemistry. Representative photomicrographs taken at 200-X magnification of uterus (A) and 400-X magnification of vagina (B) and mammary gland (C) sections from each treatment group are shown: (I) Untreated ovariectomized (OVX) rat; (II) sham-operated rat; (III) OVX rat treated with estradiol valerate (EV); and OVX rat treated with (IV) 0.7 g/kg, (V) 1.4 g/kg, and (VI) 2.8 g/kg QYFE. Data are the mean standard deviation (SD) of samples from 10 rats. **P<0.01, ***P<0.001, compared with sham; #p<0.05, ##p<0.01, ###p<0.001, compared with OVX group;△p<0.05, △△p<0.01, △△△p<0.001, compared with EV group