Research Paper Volume 11, Issue 12 pp 4032—4049

Long-time qingyan formula extract treatment exerts estrogenic activities on reproductive tissues without side effects in ovariectomized rats and via active ER to ERE-independent gene regulation

Figure 6. The effects of QYFE on the expression of estrogen receptor (ER) α and estrogen receptor (ER) β at protein levels in uterus, vagina, and mammary gland of rats. (A) Western blot analysis was carried out as described in Materials and Methods. Representative blots are shown above, and quantitative analysis is shown below. Values given are the mean standard deviation (SD) of three independent experiments. *** p < 0.001, and ** p < 0.01, compared with sham-operated; ###p<0.001, ##p< 0.01 and #p< 0.05, compared with the ovariectomized (OVX) group; (▲) p< 0.05, compared with ERα protein relative increase to Control. (B) Comparative Statistical analysis of ERα and ERβ upregulation to OVX group. (▲) p< 0.05, compared with ERα protein relative increase of QEFE to untreated OVX.