Figure 2. TRPM7 expression is downregulated in PASMCs treated with PAH stimuli in vitro (A–D) PASMCs from rats were serum starved for 24 h, followed by incubation with medium alone (control) or containing 5% fetal calf serum (FCS) (A), 100 ng/ml IGF-1 (B), 20 ng/ml TNF-α (C), or 50 ng/ml IL-6 (D) for increasing time periods as indicated. The protein level of TRPM7 was determined by Western blot analysis. β-actin was used as a loading control. The representative images (left) and band intensity analysis (right) are shown. Results are representative of 3 independent experiments. Data are mean ± SD. One-way ANOVA test. **, P < 0.01; *, P < 0.05; NS, not significant, as compared to control.