Research Paper Volume 11, Issue 12 pp 4050—4065

Figure 3. PAH stimuli reduce TRPM7 currents and intracellular free Mg2 concentration in PASMCs in vitro. (A–B) PASMCs from rats were serum starved for 24 h, followed by incubation with medium alone (control) or containing 5% fetal calf serum (FCS) for 12 or 24 h. (A) TRPM7 currents were recorded using the whole-cell patch-clamp technology with ramp from -100 mV to 100 mV. The TRPM7 currents density (pA/pF) with ramp at 100 mV is shown. Fifty cells were analyzed in each treatment. Data are mean ± SD. One-way ANOVA test. **, P < 0.01 compared to control. (B) Intracellular free Mg²⁺ concentration was determined. Data are mean ± SD from 3 independent experiments. One-way ANOVA test. **, P < 0.01 compared to control. (C–D) PASMCs from rats were serum starved for 24 h, followed by incubation with medium alone (control) or containing 100 ng/ml IGF-1 for 12 or 24 h. The TRPM7 currents density (C) and intracellular free Mg²⁺ concentration (D) were determined and analyzed as in (A–B).