Research Paper Volume 11, Issue 12 pp 4125—4144

Altered FoxO1 and PPARγ interaction in age-related ER stress-induced hepatic steatosis

Figure 5. FoxO1 regulates ER stress through PPARγ in FoxO1-virus treated cells. (A) Activation of PPARγ by FoxO1. AC2F cells were grown to 80% confluence in 100 mm dishes in DMEM, and then stimulated with 100 and 200 MOI FoxO1 and analyzed by western blotting using the appropriate antibody. (B) FoxO1-induced activation of ER stress genes. Western blot was used to detect p-IRE, total-IRE, p-PERK, and total-PERK in cytoplasmic extracts (20 μg protein) from AC2F cells. (C) AC2F cells were grown to 80% confluence in 100-mm dishes containing DMEM, pretreated (one day) with or without PPARγ-siRNA (10 nM), then stimulated with the FoxO1 virus (200 MOI) for 1 days, and Western blot was used to detect PPARγ, p-IRE, total-IRE, p-PERK, and total-PERK in cytoplasmic extracts (20 μg protein) by using the β-actin as a control from AC2F cells.