Research Paper Volume 11, Issue 12 pp 4125—4144

Altered FoxO1 and PPARγ interaction in age-related ER stress-induced hepatic steatosis

Figure 7. Effect of high glucose and FoxO1 deletion on the regulation of lipid metabolism. (A) Western blot was used to detect FoxO1 and PPARγ in FoxO1-siRNA treated liver cells. β-actin was used as a loading control. (B) The expression of SREBP-1c, PPARγ, FASN, SCD, PPARα, CPT1, and ACOX was analyzed by qPCR after treatment with glucose (30 mM) for 24 h in FoxO1-siRNA transfected (200 MOI) cells. The results were normalized based on the actin level. (C) Possible mechanism by which FoxO1 activates ER stress-induced lipogenesis in aging.