Research Paper Volume 11, Issue 12 pp 4145—4158

Intestinal stem cells acquire premature senescence and senescence associated secretory phenotype concurrent with persistent DNA damage after heavy ion radiation in mice

Figure 1. Fluorescence activated cell sorting strategy to acquire EGFP expressing Lgr5+ ISCs. Initially gates were applied using side scatter and forward scatter parameters to exclude debris and doublet cells. Subsequent gating allowed removal of dead cells and acquisition of EGFP positive ISCs for further processing.