Research Paper Volume 11, Issue 12 pp 4183—4197

Alpha-ketoglutarate extends Drosophila lifespan by inhibiting mTOR and activating AMPK

Figure 1. Effect of AKG on Drosophila lifespan. (A) Effects of low AKG concentrations on Drosophila lifespan. Treatment with low-dose AKG (0.5, 1, 5, and 10 μM) extended the lifespan of WDah females compared to control flies on 1x sugar/yeast/agar (SYA) food (1 μM vs 0 μM AKG, p < 0.001; 5 μM vs 0 μM AKG, p < 0.001; 10 μM vs 0 μM AKG, p = 0.002; log-rank test). (B) Effects of different concentrations of AKG on Drosophila median lifespan. (C) Food consumption measurement. There was no difference in food consumption between control and AKG-treated groups (ns = nonsignificant, p > 0.05, two-tailed Student’s t-tests).