Figure 3AB. TCQA enhanced β-catenin expression in the hair follicle. (A) Immunohistochemistry was performed to measure β-catenin expression in the hair follicle and the epidermis in skin collected from the treated area from mice dorsal skin at 30 days after treatment. The figure is divided into four panels, the first panel is the phase, the second is DAPI to stain the nucleus, the third is for β-catenin staining, and the last panel is a merge between β-catenin and the nucleus. (B) Ctnnb1 mRNA relative expression was measured after treatment with TCQA at 30 days after treatment. The mRNA level was quantified using TaqMan real-time PCR from RNA extracted from the treated area (TCQA or milli-Q water) from the mice dorsal back.