Research Paper Volume 11, Issue 12 pp 4254—4273

Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

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Figure 3. MR successfully increases CLS despite defective sulfur assimilation. CLS measured under regular (blue line) and MR (orange line) conditions in wild-type (a), met1Δ (b), met3Δ (c), met5Δ (d), and met10Δ (e) strains. The horizontal dotted line represents 50% viability. The graph indicates mean ± SEM. Statistical p-values between median CLS in regular and MR condition were calculated using two-tailed Student’s t-test. (f) For each strain, we plotted the change in hydrogen sulfide levels (x-axis) and change in CLS (y-axis) under MR conditions against regular condition. The Pearson’s correlation coefficient (r) was observed to be 0.01.