Research Paper Volume 11, Issue 12 pp 4254—4273

Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

Figure 3. MR successfully increases CLS despite defective sulfur assimilation. CLS measured under regular (blue line) and MR (orange line) conditions in wild-type (a), met1Δ (b), met3Δ (c), met5Δ (d), and met10Δ (e) strains. The horizontal dotted line represents 50% viability. The graph indicates mean ± SEM. Statistical p-values between median CLS in regular and MR condition were calculated using two-tailed Student’s t-test. (f) For each strain, we plotted the change in hydrogen sulfide levels (x-axis) and change in CLS (y-axis) under MR conditions against regular condition. The Pearson’s correlation coefficient (r) was observed to be 0.01.