Research Paper Volume 11, Issue 12 pp 4254—4273

Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

Figure 4. Increase in exogenous H2S does not affect cellular lifespan. Lifespan after addition of 50 μM sodium hydrosulfide (NaHS) at culture starting points (orange line) in wild-type, met3Δ, and met5Δ strains under regular (a to c) and MR (d to f) conditions. The graph indicates mean ± SEM. The gray dotted line indicates CLS without NaHS for each strain shown in Fig. 3. Statistical p-value between median CLS before and after NaHS addition was calculated using two-tailed Student’s t-test. The hydrogen sulfide levels measured using lead acetate paper is shown in the corner of the graph (original images are provided in Fig. S7).