Research Paper Volume 11, Issue 14 pp 4858—4875

CCAT1 promotes triple-negative breast cancer progression by suppressing miR-218/ZFX signaling

Figure 1. CCAT1 is upregulated in human TNBC cells and promotes proliferation, migration, and invasion in vitro. (A) Quantitative RT-PCR analysis of relative CCAT1 expression in human TNBC and adjacent normal tissue. (B) Quantitative RT-PCR analysis of relative CCAT1 expression in three TNBC cell lines (MDA-MB-231, MDA-MB-436, and MDA-MB-468) and in a normal human breast epithelial cell line (MCF-10A). (C) Quantitative RT-PCR analysis of relative CCAT1 expression in TNBC cells transfected with either si-CCAT1 or si-control. (D, E) CCK8 assays of cell proliferation following transfection of TNBC cells with either si-CCAT1 or si-control. (F, G) Analysis of the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells following transfection with either si-CCAT1 or si-control. (H, I) Analysis of the migration capacity of MDA-MB-231 and MDA-MB-468 cells following transfection with either si-CCAT1 or si-control using wound healing assays. Scale bars, 200 μm. *P < 0.05 compared to controls.