Research Paper Volume 11, Issue 14 pp 4858—4875

CCAT1 promotes triple-negative breast cancer progression by suppressing miR-218/ZFX signaling

Figure 2. Overexpression of CCAT1 promotes TNBC cell proliferation, migration, and invasion. (A) Quantitative RT-PCR analysis of relative CCAT1 expression in TNBC cells transfected with either pcDNA-CCAT1 or pcDNA-control. (B, C) Analysis of TNBC cell proliferation following transfection with either pcDNA-CCAT1 or pcDNA-control using CCK8 assays. (D, E) Analysis of the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells transfected with pcDNA-CCAT1 or pcDNA-control using transwell assays. (F, G) Analysis of the migration capacity of MDA-MB-231 and MDA-MB-468 cells transfected with pcDNA-CCAT1 or pcDNA-control using wound healing assays. *P < 0.05 compared to controls.