Research Paper Volume 11, Issue 14 pp 4858—4875

CCAT1 promotes triple-negative breast cancer progression by suppressing miR-218/ZFX signaling

Figure 4. Overexpression of miR-218 suppresses TNBC cell proliferation, migration, and invasion. (A) Relative miR-218 expression in TNBC cells following transfection with a miRNA mimic or miR-218 mimic control. (B) Analysis of TNBC cell proliferation following transfection with a miR-218 mimic or miRNA mimic control using CCK8 assays. (C, D) Analysis of the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells following transfection with a miR-218 mimic or miRNA mimic control using transwell assays. (E, F) Analysis of the migration capacity of MDA-MB-231 and MDA-MB-468 cells following transfection with either a miR-218 mimic or miRNA mimic control using wound healing assays. Scale bars, 200 μm. *P < 0.05 compared to controls.