Research Paper Volume 11, Issue 14 pp 4858—4875

CCAT1 promotes triple-negative breast cancer progression by suppressing miR-218/ZFX signaling

Figure 5. CCAT1 promotes TNBC cell proliferation, migration, and invasion by inhibiting miR-218. (A) Quantitative RT-PCR analysis of relative miR-218 expression in TNBC cells following transfection with control, si-CCAT1, or si-CCAT1 + a miR-218 inhibitor. (B, C) CCK8 assays of MDA-MB-231 and MDA-MB-468 cell proliferation following transfection with either control, si-CCAT1, and si-CCAT1 + a miR-218 inhibitor. (D, E) Analysis of the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells transfected with control, si-CCAT1, or si-CCAT1 + a miR-218 inhibitor using transwell assays. (F, G) Analysis of the migration capacity of MDA-MB-231 and MDA-MB-468 cells transfected with control, si-CCAT1, or si-CCAT1 + a miR-218 inhibitor using wound healing assays. *P < 0.05 compared to controls.