Research Paper Volume 11, Issue 14 pp 4858—4875

CCAT1 promotes triple-negative breast cancer progression by suppressing miR-218/ZFX signaling

Figure 7. MiR-218 inhibits TNBC cell proliferation, migration, and invasion by inhibiting ZFX. (A) Representative western blot analysis of TNBC cells following transfection with a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX. (B) Western blot analysis of the relative ZFX protein expression in TNBC cells following transfection with a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX. (C) Relative expression of ZFX mRNA in TNBC cells following transfection with a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX. (D, E) Analysis of MDA-MB-231 and MDA-MB-468 cell proliferation following transfection with either a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX using CCK8 assays. (F, G) Analysis of the migration and invasion capacities of MDA-MB-231 and MDA-MB-468 cells transfected with a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX using transwell assays. (H, I) Analysis of the migration capacities of MDA-MB-231 and MDA-MB-468 cells transfected with a miRNA mimic control, miR-218 mimic, or miR-218 mimic plus oeZFX. *P < 0.05 compared to controls.