Research Paper Volume 11, Issue 14 pp 4990—5007

Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2

Figure 3. FALEC overexpression inhibits the proliferation and migration of TSCC cells. (A) RT-qPCR analysis of FALEC was determined in FALEC-overexpression and control TSCC cell lines. (B) CCK-8 assays were used to determine the viability of FALEC-overexpressed TSCC cell lines. (C) EdU assays was performed to the proliferation of cell lines when FALEC was overexpressed. (D) Colony formation assays were used to determine the colony-forming ability of FALEC-overexpressed cells. Representative images (left) and average number of colonies (right) are shown. (E) Transwell assays showed that FALEC overexpression suppressed TSCC cell migration. Representative images (left) and average number of cells (right) are shown. Data are shown as means ± SD. *p < 0.05, **p < 0.01.