Figure 3. Effect of AK005401 on cell viability and apoptosis. HT22 cells were divided in four groups: control, OGD/R, AK005401siRNA, and NC. Cell viability was detected by MTT method. Cell apoptosis of all groups was observed by applying TUNEL apoptosis assay kit and AnnexinV/PI apoptosis assay kit, respectively. (A) apoptotic cells were visualized by TUNEL staining (red). Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue). (B) TUNEL positive apoptotic cells were counted as a percentage of the total number of cells. (C) Cell activities were evaluated using AnnexinV/PI apoptosis assay kit. (D) Apoptotic cells obtained from AnnexinV/PI method were counted as a percentage of the total number of cells. (E) Cell viabilities of all groups were measured by using MTT methods. Data were presented as mean±SD (n = 3 for apoptosis, n = 10 for cell viability). One-way ANOVA test was used to determine statistical significance. **P < 0.01 vs. control group, ##P < 0.01 vs. OGD/R group.