Research Paper Volume 11, Issue 14 pp 5108—5123

Activation of AK005401 aggravates acute ischemia/reperfusion mediated hippocampal injury by directly targeting YY1/FGF21

Figure 6. Effect of AK005401 on target proteins expressions. Mice and HT22 cells were used to establish I/R model and OGD/R model, respectively. The expression levels of YY1, FGF21, PI3K, Akt, caspase 3, Bcl-2, and Bax were visualized using chemiluminescence method. As shown in Figure 6, (A) represents Western blots in panel (1, 2, 3, and 4 represent sham, I/R, AK005401siRNA, and NC, respectively). (A1–A6) represent the relative expressions of YY1, FGF21, PI3K, Akt, caspase 3, and the relative ratio of Bax/Bcl-2 in hippocampus tissue, respectively. As shown in Figure 6-2, (B) represents Western blots in panel (1, 2, 3, and 4 represent control, OGD/R, AKsiRNA, and NC, respectively). (B1–B6) represent the relative expressions of YY1, FGF21, PI3K, Akt, caspase 3, and the relative ratio of Bax/Bcl-2 in HT22 cells, respectively. Data were presented as mean±SD (n = 3). One-way ANOVA test was used to determine statistical significance. *P < 0.05 or **P < 0.01 vs. sham group or control group, ##P < 0.01 vs. I/R group or OGD/R group.