Figure 2. Detection of AGE formation after glycation. THP-1 macrophages (M0) were incubated with different concentrations of MGO for 24 h. Total proteins were separated by SDS-PAGE and immunoblotted. (A) Formation of AGEs was detected using an anti-AGE antibody (CML-26). The depicted blot represents 3 independent experiments. (B) Corresponding Ponceau staining of representative blot.