Figure 3. Detection of surface glycation. THP-1 macrophages (M0) were treated with or without 1 mM MGO for 24 h. Immunofluorescence staining of surface glycation was performed using an anti-AGE antibody (CML-26, shown in orange). Hoechst was used as nuclear stain (shown in blue). Shown pictures are representative for 4 independent experiments. Scale bars indicate 100 µm.