RAGE expression after glycation. THP-1 macrophages (M0) were incubated with 1 mM MGO or 10% AGE-FCS for 24 h in normal growth medium. Total protein was separated by SDS-PAGE and immunoblotting. RAGE expression was detected using an anti-RAGE antibody (ab3611); (A) and quantified in relation to actin staining (B). The Graph shows average mean of relative RAGE expression + SD of 4 independent experiments.