Figure 5. Apoptosis assay. THP-1 macrophages (M0) were treated with different MGO concentrations or 10% AGE-FCS for 24 h and apoptosis assay was performed using 7AAD and Annexin V staining. The percentage of Annexin V- / 7AAD- cells was used to determine the intact living cells (= non-apoptotic and non-necrotic). Graph shows average mean + SD of 3 independent experiments.