Figure 3. RT-PCR analysis of Dlx5 and Dlx6 expression in the cerebral cortex. (A) Primers p1 to p4 were used to analyze the presence of Dlx5 and Dlx6 transcripts in reverse-transcribed RNA extracts from adult cerebral cortex fragments. (B) Two known splice variants of Dlx5(αDlx5 and γDlx5, ) were amplified with primers p3 and p4. Deletion of exon II shifted both bands giving rise to αDlx5Δ and γDlx5Δ. A small fraction of Dlx5 transcripts, possibly corresponding to expression of this gene in non-GABAergic cells or a few non-cre expressing Vgat-positive cells, was not recombined. Dlx6 transcripts were amplified with primers p1 and p2 and did not show any splice variants.