Research Paper Volume 11, Issue 16 pp 5975—5991

LINC00511 promotes the malignant phenotype of clear cell renal cell carcinoma by sponging microRNA-625 and thereby increasing cyclin D1 expression

Figure 2. LINC00511 knockdown inhibits ccRCC proliferation and induces apoptosis of A498 and 786-O cells. (A) A498 and 786-O cells were transfected with either si-LINC00511 or si-NC. After transfection, LINC00511 expression was determined by RT-qPCR. *P < 0.05 vs. the si-NC group. (B) CCK-8 assays were performed to measure the proliferative ability of A498 and 786-O cells treated with either si-LINC00511 or si-NC. *P < 0.05 vs. group “si-NC.” (C) Colony formation assays of A498 and 786-O cells transfected with either si-LINC00511 or si-NC. Representative images for each treatment are shown. *P < 0.05 vs. the si-NC group. (D, E) Cell cycle and apoptosis assays were performed to determine the cell cycle status and apoptotic rate of A498 and 786-O cells after transfection with either si-LINC00511 or si-NC. *P < 0.05 vs. group si-NC. (F, G) Si-LINC00511 or si-NC was introduced into A498 and 786-O cells. Migration and invasion abilities were assessed by Transwell migration and invasion assays. *P < 0.05 vs. the si-NC group.