Research Paper Volume 11, Issue 16 pp 5975—5991

LINC00511 promotes the malignant phenotype of clear cell renal cell carcinoma by sponging microRNA-625 and thereby increasing cyclin D1 expression

Figure 4. miR-625 upregulation inhibits the growth and metastasis of ccRCC cells in vitro. (A, B) A498 and 786-O cells transfected with the miR-625 mimics or miR-NC were collected. These cells were subjected to CCK-8 and colony formation assays to determine cell proliferation and colony formation capacity, respectively. *P < 0.05 vs. the miR-NC group. (C, D) Flow-cytometric determination of the cell cycle distribution and apoptotic rate of miR-625–overexpressing A498 and 786-O cells. *P < 0.05 vs. group miR-NC. (E, F) The impact of miR-625 overexpression on A498 and 786-O cell migration and invasion was determined in Transwell migration and invasion assays, respectively. Representative images and quantification are presented. *P < 0.05 vs. group miR-NC.