Research Paper Volume 11, Issue 16 pp 5975—5991

LINC00511 promotes the malignant phenotype of clear cell renal cell carcinoma by sponging microRNA-625 and thereby increasing cyclin D1 expression


Figure 8. Reduction in LINC00511 expression inhibits ccRCC tumor growth in vivo. (A) Representative images of xenograft tumors derived from si-LINC00511–transfected or si-NC–transfected A498 cells. (B) Tumor volumes in the BALB/c nude mice of si-LINC00511 and si-NC groups were calculated every 2 days and growth curves were generated. *P < 0.05 vs. group si-NC. (C) The tumor xenografts in the si-LINC00511 and si-NC groups of BALB/c nude mice were excised and weighed. *P < 0.05 vs. group si-NC. (D, E) Expression levels of LINC00511 and miR-625 in xenograft tumors were quantified by RT-qPCR. LINC00511 expression was found to be decreased, while miR-625 expression was high in the xenografts of the si-LINC00511 group compared to the si-NC group. *P < 0.05 vs. the si-NC group. (F) Protein expression of CCND1 in the xenografts of si-LINC00511 and si-NC groups was determined by western blotting.