Research Paper Volume 11, Issue 16 pp 5992—6013

LncRNA RP11-670E13.6, interacted with hnRNPH, delays cellular senescence by sponging microRNA-663a in UVB damaged dermal fibroblasts

Figure 2. RP11-670E13.6 promoted DNA damage repair. (A) Top significant biological processes for genes whose transcript levels were increased in RP11-670E13.6-depleted HDFs. (B) Top significant Kyoto Encyclopedia of Genes and Genomes pathways for genes whose transcript levels were increased in RP11-670E13.6-depleted HDFs. (C) Comet tail length was quantified at 24 h after 40 mJ/cm2 UVB irradiation. Representative images are shown. Data are shown as the means ± standard errors of the means. (D) Representative image of western blotting results for the effects of RP11-670E13.6 on the expression of ATM protein in HDFs. (E) Relative expression of the indicated DNA damage-associated genes was determined by qRT-PCR in RP11-670E13.6-depleted HDFs and negative controls. Data are shown as the means ± standard errors of the means based on at least three independent experiments. (F) HDFs were mock treated or transfected with siRNA against RP11-670E13.6. Two days after transfection, the cells were UVB (40mJ/cm2) irradiated and analyzed for H2AX phosphorylation at the indicated time points by western blot. (G) HDFs were mock treated or transfected with siRNA against RP11-670E13.6. Two days after transfection, the cells were UVB (40mJ/cm2) irradiated and analyzed for H2AX phosphorylation at 24h post-irradiation by immunofluorescent staining. (H) Quantification of γH2A.X foci expressed as mean relative area per cell. Twenty nuclei from the HDFs transfected with RP11-670E13.6 siRNA and control siRNA were examined. P values were determined by Student’s t-tests. *P < 0.05; **P < 0.01; and ***P < 0.001.