Research Paper Volume 11, Issue 16 pp 5992—6013

LncRNA RP11-670E13.6, interacted with hnRNPH, delays cellular senescence by sponging microRNA-663a in UVB damaged dermal fibroblasts

Figure 3. RP11-670E13.6 cellular localization. (A) FISH images showing localization of RP11-670E13.6 in HDFs treated with or without UVB irradiation for 24 h. (B) Percentage of nuclear and cytoplasmic RNA levels of RP11-670E13.6, U6 and GAPDH measured by qRT-PCR after subcellular fractionation in HDFs irradiated or not irradiated with UVB for 24 h. Data are shown as the means ± standard errors of the means based on at least three independent experiments. P values were determined by Student’s t-tests. *P < 0.05; **P < 0.01; and ***P < 0.001. FISH, fluorescence in situ hybridization; 18S, probe for 18S rRNA; U6, probe for U6 snRNA.