Research Paper Volume 11, Issue 16 pp 6014—6028

MiR-146b accelerates osteoarthritis progression by targeting alpha-2-macroglobulin

Figure 1. miR-146b expression is up-regulated in OA-damaged cartilage tissues and in IL-1β-stimulated mouse chondrocytes. (A) Representative macromorphological images of smooth cartilage and damaged cartilage from OA patients. (B, C) Safranin O-fast green and H&E staining of smooth OA cartilage and damaged OA cartilage. Scale bar: 100μm. (D) The expression level of miR-146b in smooth and damaged cartilage tissues. (E) Time course of the miR-146b expression under IL-1β stimulation in mouse chondrocytes. (F) mRNA level of miR-146b in mouse chondrocytes transfected with miR-146b-mimic and miR-146b-inhibitor were evaluated by qRT-PCR. The negative control of miR-146b mimic and miR-146b inhibitor were referred as to scramble and NC. *P<0.05, **P<0.01, ***P<0.001.