Research Paper Volume 11, Issue 16 pp 6014—6028

MiR-146b accelerates osteoarthritis progression by targeting alpha-2-macroglobulin

Figure 5. Inhibition of miR-146b in cartilage delays the progression of osteoarthritis in mice. (A) After DMM surgery or sham operation, mice were intra-articularly injected with 250 μM antago-miR-146b or antago-miR NC on day 7 and day 14 after surgery. Knee joints were harvested at 5 weeks after last injection and analysed histologically by Safranin O-fast green staining. (B) OARSI scores based on safranin O and fast green staining in (A). (C) Quantification of HC/CC according to H&E staining in (A). (D) Immunostaining analysis of positive A2M. (E) qRT-PCR analysis of miR-146b levels in mice knee joint cartilage. Scale bar: 100μm (first line); 50μm (others). *P<0.05.